Ambiguous sequences at different cut off levels - (Item E)

For the evaluation of ambiguous sequences in microbial reference genomes we assessed different cut of levels for the generation of a curated microbial genome database. For
each sequence fragment we determined the ratio between the number of negative
BLAST hits (query genus ≠ subject genus) and all BLAST hits.

For a detailed description of this analysis, see the Supplemental Materials section of the publication.

Reference

Kirstahler P,
Bjerrum SS, Friis-Møller A, la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018) Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid. Scientific
Reports, doi:10.1038/s41598-018-22416-4.