TIRF and PREM correlative images of PC12 cells supporting data in figure 1 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>It contains the raw images used in fig1a. where representative TIRF images for NPY-mNeongreen and Rab27a-mCherry labeled PC12 cells are shown. It contains raw EM and TIRF images used for correlative TIRF/EM in fig. 1c. And, it contains all the underlying raw tirf images used in the correlative analysis shown in fig. 1b. <br>For TIRF correlative experiment, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG or NPY-mCherry and GFP, mcherry or mRFP tagged proteins of interests. Cells were rinsed in intracellular buffer and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde. And TIRF imaging performed using an Olympus IX-81 inverted fluorescence microscope.