RNAseq Analysis of Transcriptome expression profiles in E12.5 and E13.5 FACS sorted Osr2RFP/- (mutant)and Osr2RFP/+ (control) palatal mesenchymal cells

Purpose: The purpose of this study is to compare the transcriptome expression profiles of E12.5 and E13.5 Osr2RFP/- and Osr2RFP/+ palatal mesenchyme by using RNA-seq analysis. Methods: Osr2RFP/+ male mice were crossed with Osr2+/- female mice. The embryos were harvested at E12.5 and E13.5. The pair of palatal shelves were dissected from each Osr2-RFP positive embryo. The RFP+ palatal mesenchyme cells were isolated by using fluorescence-activated cell sorting (FACS). RNA-seq analysis was carried out using the FACS-isolated palatal mesenchyme from Osr2RFP/- and Osr2RFP/+ embryos, respectively. The transcriptome expression profiles of E12.5 and E13.5, Osr2RFP/- and Osr2RFP/+ palatal mesenchyme by using RNA-seq analysis, in single pair (E12.5) or duplicates (E13.5), using Illumina HisEq 2000.研究目的：本研究旨在通过RNA测序（RNA-seq）分析，对比胚胎发育第12.5天（E12.5）与第13.5天（E13.5）时期，Osr2RFP/-及Osr2RFP/+小鼠腭间充质的转录组表达谱。 实验方法：将Osr2RFP/+雄性小鼠与Osr2+/-雌性小鼠交配，分别于E12.5和E13.5阶段采集胚胎。从每一枚Osr2-RFP阳性胚胎中分离双侧腭板，通过荧光激活细胞分选（FACS）技术分离得到RFP阳性的腭间充质细胞。分别采用来自Osr2RFP/-与Osr2RFP/+胚胎的FACS分选腭间充质细胞开展RNA-seq分析；其中E12.5时期样本设置为单份重复，E13.5时期样本设置为双份重复，测序平台采用Illumina HisEq 2000。