N-hydroxy-pipecolic acid mediated priming of salicylic acid signalling in Arabidopsis thaliana wrky38/wrky62 double mutants

N-hydroxy-pipecolic acid (NHP) primes Aarabidopsis thaliana to confer faster and stronger activation of immune responses, in part by priming salicylic acid (SA) signalling. Here we investigated the potential regulators of NHP-SA crosstalk in priming. We show that in wrky38/wrky62 double mutants, SA signalling is minimally affected but the NHP priming effect is greatly attenuated, with most wild type primed genes (identified in E-MTAB-14631) no longer NHP responsive. 12 day old wrky38 (SAIL_749_B02)/wrky62 (SM_3_38820) double mutant seedlings grown on MS media were treated with 1mM NHP or water overnight, then 0.5mM SA or water for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.