Effect of sterol treatment on membrane composition and structure.

A. Mean±standard deviation (3 experiments) of total sterol contents (CH+7KC) of JCaM2 and Jurkat 8.2 cells are given in nmol/mg cell protein. 7KC levels are a percentage of total sterol levels. B. Mean±standard deviation (of n images) of GP values at contact sites between JCaM2 cells and beads after 7 min of activation and between Jurkat 8.2 cells and B cells in the presence or absence of antigen after 7 min and 23 min of activation. Statistically significant differences between activation and control sites are indicated with ** P<0.001, * P<0.05. C. Global GP values of JCaM2 cells after 7 min of activation. Normalized GP distributions were fitting to two Gaussian populations (fluid and ordered). The mean GP value (and coverage) of each population is given.