Transcriptome and epigenome data analysis to reveal the key role of KAKU4 during leaf senescence in Arabidopsis. Transcriptome and epigenome data analysis to reveal the key role of KAKU4 during leaf senescence in Arabidopsis

We conducted the combination analysis of transcriptomic and epigenomic data between kaku4-2 mutant and WT suggested that KAKU4 may inhibit the expression of a series of genes related to hormone signals and H2O2 metabolism by affecting the deposition of H3K27me3, thereby suppressing leaf senescence. Overall design: Rosette leaves of the 6-week-old kaku4-2 mutant and WT plants were sampled for RNA-seq with three independent biological replicates. The ChIP-seq analyses of H3K27me3 were conducted using the 6-week-old rosette leaves of kaku4-2 mutant and WT.