Complete genome analysis of a novel picorna-like virus from a ladybird beetle, Cheilomenes sexmaculata

The ladybird beetle Cheilomenes sexmaculata , is a common insect predator of agricultural pests. In this study, the full genome sequence of a novel picorna-like virus, temporarily named Cheilomenes sexmaculata picorna-like virus 1, was identified from C. sexmaculata. The full-length sequence of CSPLV1 was 11,384 nucleotide in length with one predicted open reading frame encoding 3727 amino acids, a 13 nt 5' untranslated region and a 187 nt 3' UTR. The ORF of CSPLV1 consisted of four distinct domains including an RNA virus helicase domain , a peptidase domain, an RNA-dependent RNA polymerase domain and a picorna like coat protein domain. Phylogenetic analysis based on the conserved RdRP sequence showed that CSPLV1, together with Wuhan house centipede virus 3, Hypera postica associated virus 1 and Diabrotica undecimpunctata virus 1, formed as an unclassified group which is closely related to the clade Solinviviridae. To the best of our knowledge, CSPLV1 is the first picorna like virus revealed in C. sexmaculata.