Effect of BRCA1 overexpression on genetic expressions of cervical cancer based on next generation sequencing

The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (Sangon, China) and 1% antibiotic-antimitotic solution (Gibco, USA). The SiHa cells were transfected using Lipofectamine 3000 reagent following the manufacturer's protocol and incubated for 48h for further analysis.The gene expression profiles were analyzed of SiHa cells transfected with GV230-BRCA1 or GV230 plasmid to assess effect of BRCA1 overexpression on genetic expressions of cervical cancer. Overall design: High-throughput sequencing raw data for 6 samples, A1;A2;A3 are three samples from experimental group (SiHa cells with GV230-BRCA1 plasmid transfection),and C1;C2;C3 are three samples from negative control group (SiHa cells with GV230 plasmid transfection).