C. elegans small RNA-seq profiling of three different lotr-1 mutant alleles

Proteins containing LOTUS and Tudor domains have critical roles in the germline, including in small RNA biology. LOTR-1 is the only protein expressed in the germline of C. elegans which has LOTUS and Tudor domains. Here, to understand the influence of LOTR-1 in small RNA biology, we sequenced small RNAs of wild-type and lotr-1 mutant animals. Small RNA-seq profiling of wild-type and lotr-1 mutant worms. The three mutants analysed in this experiment have different deletions in the lotr-1 gene (details are described in the associated paper). RNA was treated with RppH prior to library preparation.