A trivalent nucleosome interaction by PHIP/BRWD2 is disrupted in neurodevelopmental disorders and cancer

The PHIP chromatin reader protein is mutated in Chung-Jansen syndrome, a neurodevelopmental disorder with autism like symptoms. Here we have characterized the chromatin binding properties of PHIP using a variety of in vitro and in vivo methods. Overall design: We have performed ChIP-sequencing of histone modifications identified as candidate binding sites for the PHIP Tudor and Bromo domains, test to if they co-occur with PHIP occupancy. In addition we have performed RNA-sequencing of PHIP loss-of-function mutants.