RNA-Seq of BW25113 Escherichia coli (wild-type and a mutant lacking yeaG) experiencing initial and sustained nitrogen starvation in Gutnick minimal media. RNA-Seq of BW25113 Escherichia coli (wild-type and a mutant lacking yeaG) experiencing initial and sustained nitrogen starvation in Gutnick minimal media

RNA-seq was carried out to compare the transcriptomes of wild-type BW25133 E coli with a mutant lacking the conserved serine/threonine kinase, YeaG, with the aim of understanding how YeaG contributes to the transcriptional response to sustained nitrogen starvation, and identify pathways affected by YeaG. We also compare the wild-type transcriptome during nitrogen-replete, following nitrogen starvation, and under 24 h of sustained nitrogen starvation to identify pathways involved in the response to nitrogen run out, and also in adaptation to sustained nitrogen starvation.