Single worm DNAseq for hybrid male inviability

Wild-type C. briggsae and C. nigoni can produce fertile hybrid XX female progeny, but XO progeny are either 100% inviable (when C. briggsae is the mother) or viable but sterile (when C. nigoni is the mother). As F1 male progeny of C. briggsae (father) X C. nigoni ZZY10337 (mother) crosses are fertile, we can use these F1 males to screen for specific ChrX-autosome interactions that might contribute to hybrid male inviability. To this end, we crossed fertile F1 males with C. briggsae hermaphrodites, then collected the resulting viable B2 males for genotyping by single-worm genome sequencing