Translocation of WmCSV and TYLCV into the hemolymph of the efficiently transmitting Bemisia tabaci 63 and the poor transmitter B. tabaci 95.

Hemolymph was collected 0, 2, 4, 6, 8, 24, 30 h and 6 d after transfer of starved whiteflies to infected plants. Virus concentrations in pooled hemolymph of 5 females was analyzed by qPCR in a relative approach with 18S rDNA as reference gene (n = 3, with two technical replicates). ΔCq data of the relative quantification are displayed reciprocally for better illustration. Standard curves of both viruses were included to allow an estimation of absolute virus concentration under the assumption that 10 and 80% of hemolymph was extracted, respectively. Asterisks indicate significant differences between the whitefly populations for one virus at a given time point; Student's t-test, pTranslocation of WmCSV and TYLCV into the hemolymph of the efficiently transmitting Bemisia tabaci 63 and the poor transmitter B. tabaci 95.