Single turnover DNA cleavage rate constants (kobs) of 1 nM 32P labeled 18–1 DNA, 1 μM SgrAI enzyme dimer mutant and varied concentration of unlabeled PC DNA.

aValues presented at the average of three independent trials ± the standard deviation.bPercent Cleaved is the percentage of DNA cleaved by SgrAI in the assay by the given rate constant (kobs). In cases where less than 100% is cleaved by the given rate constant, the remainder is cleaved by a nonaccelerated rate constant.cRelative Acceleration is the ratio of kobs in the presence of 1 μM PC DNA to that in the absence of added PC DNA.Single turnover DNA cleavage rate constants (kobs) of 1 nM 32P labeled 18–1 DNA, 1 μM SgrAI enzyme dimer mutant and varied concentration of unlabeled PC DNA.