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Two primer sets targeting phoD alkaline phosphatase were compared using 454-sequencing on six grassland soils.

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https://www.ncbi.nlm.nih.gov/sra/ERP008947
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资源简介:
To date, one primer set has been designed for phoD alkaline phosphatase in soil. However, these primers have been reported to have an amplification bias towards Alphaproteobacteria. This leads to a lower coverage of the large phoD diversity. This study consists of the comparison of the published primers with newly-designed primers targeting phoD alkaline phosphatase using 454-sequencing on six grassland soils.
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2021-02-04
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