scRNA-seq analysis of the effect IL20RB knock-out in esophageal immune cells of an eosinophilic esophagitis (EoE) mouse model

An EoE mouse model was induced by epicutaneous sensitization and oral challange with ovalbumin. Single-cell RNA-seq was performed to compare CD45+ immune cells from wild-type and IL20RB knock-out mice esophagus samples. Overall design: Esophagus cell suspensions from five wild-type animals were labeled with BioLegend hashtaging antibodies TotalSeq-B0301 to B0305, while suspensions from five IL20RB knock-out animals were labeled with antibodies TotalSeq-B0306-B0310. All 10 cell suspensions were pooled and sorted for live CD45+ cells. The pooled single cell suspension was loaded on 2 wells from a cartridge of the 10X Genomics Chromium Single Cell Controller for cell capture and library preparation.