Secondary structure and backbone relaxation analysis of MPS-1(134–256) in the absence or presence of DPC micelles.

(A) TALOS + secondary structure prediction were based on the assigned backbone 13CO, 13Cα, 13Cβ chemical shifts of MPS-1(134–256) in the presence or absence of DPC. Only random coil secondary structure was observed for MPS-1(134–256) in both aqueous buffer and DPC micelles. (See Fig. S3 in file S1 for the Y-axis scale from 0 to 0.3) (B) Distribution of the measured 15N T1 longitudinal and T2 transverse relaxation times and steady-state 1H-15N NOE values along the primary sequence in the absence (black) or presence (red) of DPC micelles.