Human bladder cancer cell line high throughput sequencing

Circular RNAs (circRNAs) have been increasingly indicated to be important participants in the development and progression of various malignant tumors. In this study, we analyzed circRNA high-throughout sequencing from human bladder cancer tissues and paired adjacent normal tissues. Then we focused on the upregulated circRNAs and identified a novel circular RNA, hsa_circ_0008399 , as a new candidate circRNA derived from RBM2 gene. FISH assay has been proved that hsa_circ_0008399 mainly located in nucleus. Transcriptome analysis was performed to find the potential genes that could be regulated by hsa_circ_0008399. Overall design: In order to screen downstream genes regulated by hsa_circ_0008399, transcriptome analysis was performed in EJ and T24T cells transfected with has_circ_0008399 and compaired vector.