Trihelix transcription factor SlGT31 regulates fruit ripening mediated by ethylene in tomato

Trihelix proteins are plant-specific transcription factors that play crucial roles in plant development and stress responses. However, the involvement of trihelix proteins in fruit ripening and transcriptional regulatory mechanisms remains largely unclear. In this study, we cloned a trihelix gene SlGT31, whose relative expression was significantly induced by the application of exogenous ethylene but repressed by 1-methylcyclopropene (1-MCP). Suppression of SlGT31 resulted in delayed fruit ripening, decreased accumulation of total carotenoids and ethylene content, and inhibition of relative expression of genes related to ethylene and fruit ripening. Conversely, the opposite results were observed in SlGT31-overexpression lines. Yeast one-hybrid and dual-luciferase assays suggested that SlGT31 could bind to the promoters of two key ethylene biosynthesis genes ACO1 and ACS4. These results indicate that SlGT31 may act as a positive modulator during fruit ripening., Plant Materials and Growth Conditions
Tomato (Solanum lycopersicum Mill.var. Ailsa Craig) was used as the wild type (WT) in this study. WT and transgenic lines were grown in a glasshouse under controlled conditions with 16-h-light/8-h-dark cycles, 25°C-day/18°C-night temperatures, 80% relative humidity, and 250 μmol m−2 sec−1 luminous intensity. Flowers were tagged at the anthesis stage, immature green was defined as 20 DPA (days post-anthesis), and mature green was defined as 35 DPA. Breaker fruits were defined as fruits of 38 DPA with the color starting to generate a slight yellow color. Other fruits from the 4th and 7th days after breaker were also used. Fruits at different ripening stages were collected, frozen immediately in liquid nitrogen, and stored at -80°C until use.
Sequence analysis and subcellular localization
For SlGT31-GFP construction, the cDNA with the termination codon removed from SlGT31 was amplified with SlGT31-GFP-F/R primers (Supplementary Table.S1). The amplified..., , ## Trihelix transcription factor SlGT31 regulates fruit ripening mediated by ethylene in tomato

[https://doi.org/10.5061/dryad.vdncjsz18](https://doi.org/10.5061/dryad.vdncjsz18)

### Description of the data and file structure

In this study, all tomato plants were grown in a glasshouse under controlled conditions with 16-h-light/8-h-dark cycles, 25°C-day/18°C-night temperatures, 80% relative humidity, and 250 μmol m−2 sec−1 luminous intensity. The independent overexpression lines and RNA interference (RNAi) lines were obtained through genetic transformation mediated by *Agrobacterium tumefaciens* strain LBA4404. All data are means ± standard deviation of at least three independent experiments. A single asterisk (\*) and double asterisks (\*\*) in the figures indicate significant differences of *P* < 0.05 and significant differences of *P* < 0.01, respectively. These datasets contain the mean and standard deviation (SD) of the data corresponding to the experiments and figures in ...