Anticoronavirus Activity of Uridine Glycoconjugates Containing a 1,2,3-Triazole Moiety - Dataset

<p>The dataset presents the results of several experiments. An MHV cytopathic effect reduction assay was used to evaluate the antiviral activity of compounds 14–25 (Fig. 2A). Both the protective efficacy of each compound (50 μM) against MHV infection and its cytotoxicity in uninfected cells were assessed using an MTS-based proliferation assay. The antiviral activity of the compounds against HCoV-NL63 was determined by analyzing nucleocapsid (N) protein levels via Western blot, followed by densitometric analysis (Fig. 2C). The dose-dependent effects of selected compounds against MHV and HCoV-NL63 were evaluated by measuring virus titers in the culture medium of infected cells (Fig. 3B and 3D). The SARS-CoV-2 replicon system in the BHK-21 cell line was used to assess the effects of the analyzed compounds on viral replication, in comparison with nirmatrelvir, a known 3C-like protease inhibitor that blocks viral replication (Fig. 5C).</p>