Role of Dot6 in size control in Candida albicans

<br><b>Figure S1. </b><b>A <i>sch9</i>mutant adjusts cell size in response to different carbon sources.</b><b></b>Size distribution of log-phase cultures of the indicated WT (CAI4) and <i>sch9</i>strains grown in synthetic glucose (black curve) and glycerol (red) medium.<b>Figure S2. Localization of Dot6-GFP in the vacuole when TOR pathway is compromised. </b>Dot6-GFP fluorescence was visualized using confocal microscopy in cells treated with the TOR pathway inhibitor, rapamycin. Vacuoles were stained using CellTracker Blue CMACdye. Red and blue arrows indicate Dot6-GFP florescence in the vacuole and the nucleus, respectively. <b>Figure S3. Dot6 is required for size homeostasis of hyphal cells.</b>Length of at least 20 hyphal cells of both WT (SFY87) and dot6 mutant grown on YPD supplemented with 10% fetal bovine serum (FBS) at 37°C. Bars represent the means ±standard errors of the means. *, <i>P </i>&lt; 0.0003 using a two-tailed t-test. <b> </b><b>Table S1. </b>Strains used in the current study and their genotypes.<b>Table S2</b>. Primer sequences used in the current study.<b>Table S3</b>. Gene Set Enrichment Analysis (GSEA) of dot6 mutant transcriptome <b>Table S4</b>. Transcripts differentially expressed in dot6 mutant using a 1.5-fold change cut-off and a 5% false discovery rate.