aExcess primer = X, Limiting primer = L. bF. tularensis subspecies tularensis yields amplicon of 307 bp, subspecies novicida and mediasiatica of 451 bp.
aTm was predicted using Primer Premier 5.0 software. bThe 16S rDNA based probe was used as the positive control. cThe probe containing 40 poly(T) oligonucleotides was used as the negative control. dTh
An undecamer oligonucleotide probe based on a pair of deoxythymidines flanked by several modified nucleotides is a specific and highly efficient biosensor for micrococcal nuclease (MNase), an endonucl
