Assessment of the effect of nutrition level on metylome of mammary gland structural components (parenchyma (PAR) and fat pad (MFP)) and liver in dairy calves

The aim of this study was to identify changes in metylome of parenchyma (PAR), fat pad (MFP) and liver in dairy calves. Our earlier results showed that feeding management regulates the dynamics of organ growth in preweaned heifers. We observed that a daily dose of 5.3 kg of milk replacer inhibits mammary gland development. Therefore, this research aims at evaluation of the influence of the nutritional level in the early life period of calves on the biological processes related to the development of the mammary gland through transcriptomic alterations. Overall design: Comparative DNA methylation profiling analysis of RRBS data for 14 parenchyma, 16 fat pad and 15 liver samples was performed using the Illumina Nextseq 550 platform.