3T3-L1 cell volumes and estimated triacylglycerol content after 3-day exposure to media with different glucose or fructose concentrations.

The data represent the mean ± SD of the analyses of three different (contiguous) wells with Transwell inserts. Well biomass represents the sum of volumes of all particles detected; the differences between cell volumes and total biomass is presented as well debris (as a percentage of total biomass). Cell triacylglycerols (TAG) were estimated, as described under Methods, assuming that at most 75% of the cell volume was made up of TAG. The estimations are an approximation made using only the mean values, as are the changes observed in biomass per cell (comparing the volumes of cells with those in the medium devoid of monosaccharides. The differences in cell volume (mass) were translated into estimated changes of triacylglycerol content per day. There were no statistically significant differences for cell volume, or type and concentration of monosaccharide (3-way ANOVA)3T3-L1 cell volumes and estimated triacylglycerol content after 3-day exposure to media with different glucose or fructose concentrations.