GROQ-seq Function Measurements for TEV Protease SSVL and epPCR Libraries.

This dataset contains GROQ-seq functional measurements of TEV protease variant libraries — a site-saturation variant library (SSVL) and an error-prone PCR (epPCR) library — covering over 18,000 protease variants across 24 experimental conditions. Fitness is measured via a split-DHFR system in which the TEV protease substrate is embedded as the linker of a split dihydrofolate reductase: TEV cleavage destroys DHFR and reduces trimethoprim resistance, translating protease activity into differences in cellular growth. Conditions vary independently tuned levels of TEV expression, split-DHFR expression, and trimethoprim selection. This run of the assay was performed at the Living Measurement Systems Foundry (LMSF) at the National Institute of Standards and Technology. Part of a broader effort to generate sequence → function data for proteases, enabling predictive models of protease activity and specificity with applications in protein engineering and therapeutic development.