Figure 2

TNF-α-induced NF-kB nuclear translocation in human iPSC-derived astrocytes.(a) - Photomicrographs of NF-kB immunostaining 1 h after exposing cells to vehicle or five different concentrations of TNF-α. Quantification of NF-kB immunoreactivity in both (b) – Nuclei and (c) – Whole cell area, which were expressed in arbitrary units of immunofluorescence (A.U.). (d) – The NF-kB translocation index (nuclei/whole cell area ratio). Data are presented as means ± SEM of experiments performed in triplicates from 3 cell lines. *P < 0.05, different from vehicle; **P < 0.05, different from vehicle and 1 ng/mL TNF-α. ***P < 0.05, different from 10 ng/mL TNF-α. One-way ANOVA followed Tukey´s post hoc test. Magnification: 100 x. Calibration bar: 200 μm.