Recombinant LigB proteins.
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aThe unique repeats of LigB from Leptospira interrogans Copenhageni L1-130 were subcloned in pET-20b(+) at the Nde I/Xho I site, which adds an initiating methionine and an LEH6 tail to the Lig protein. The recombinant proteins are named for their repeats; the prime designation indicates a partial repeat.
bLigB-unique repeats are residues 582 to 1118 (full repeat 7 to repeat 12). The specific repeats are 7: K582-V669, 7′: A631-V669, 8: Q673-V756, 9: L760-I845, 10: K851-F936, 11: T942-V1028, and 12: T1032-V1118. All constructs include the short 3- to 5-residue linker following the last repeat, except repeat 12, which includes seven residues into the LigB C-terminal domain (amino acids 1119 to 1890).
cincludes pET-encoded amino acids.
dalso cloned with N-terminal H6 in pET-14b at the Nde I/XhoI site, producing a protein with mol. wt. 33,174.
创建时间：
2011-02-09
