Quantitative Phosphoproteomics Reveals in vivo Boron Deficiency Induced Signaling Dynamics in Arabidopsis Roots

Here we describe an improved tandem MOAC combined phosphoprotein / phosphopeptide enrichment strategy, a scalable phosphoproteomics approach that allows rapid identification of thousands of phophopeptides in plant material. We apply this technology to generate time-resolved maps of boron signaling in Arabidopsis roots.