Renal gene expression in two mouse strains with different propensity to develop chronic kidney disease after subtotal nephrectomy at different time-points

Renal gene expression analysis was performed in mouse strains with different propensity to develop progressive chronic kidney disease (CKD) after subtotal nephrectomy: the FVB strain which is spontaneously highly predisposed to CKD and the C57BL/6 which is spontaneously not predisposed to CKD. Subtotal nephrectomy (Nx) is normally initially compensated by proliferative tissue repair (2 days after nephrectomy). After this initial proliferation follows a quiescent period (28 days after NX). Finally, specifically in the sensitive strain there is lesion onset (53 days after Nx). Gene expression was monitored on RNA from whole kidneys from different mouse strains Sham operated or Nephrectomised at three different time-points. 9 weeks old lesion-predisposed FVB mice or lesion-non-predisposed C57BL/6 mice were subjected either to Subtotal-Nephrectomy (Nx) or sham-operation (S). Gene expression was monitored at three different time points: 2, 28 and 53 days after either Subtotal Nephrectomy or Sham Operation. Gene expression was monitored on whole kidney RNA with Affymetrix microarrays.