Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells. Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells

We reported direct reprogramming human PBMCs into induced MSCs using non-integrating episomal vectors, and performed epigenetic analysis, including chromatin openness and methylation status, along with transcriptomic analysis, on functional as well as non-functional induced MSCs from different reprogramming factors combinations, in order to better understand epigenetic signature of functional MSCs. Overall design: RNA-seq, ATAC-seq, and RRBS methylation profiles of day 28 induced MSCs reprogrammed from human peripheral blood mononuclear cells by three different reprogramming factors combinations.