Response of osteoblasts on amine-based polymer coatings cor-relates with the amino group density

The integration of implants can be improved by physical but mainly by chemical modifications of the implant surface. It has already been shown that amine-based coatings improve cell attachment, cell migration, and intracellular signaling. The aim of this study was to determine the role of the amino group density in this positive cell response by developing controlled amino-rich nano-layers. Using Clariom S microarrays to perform genome-wide expression profilng, changes in adhesion related genes after cultivation of osteoblast-like cells on an amino group-rich coating could be seen. Human MG-63 osteoblast-like cells (ATCC® CRL-1427™, United States) were grown for 4 hours, respectively for 24 hours on amine-based polymer coating Ti-TMS-PEI (trimethoxysilylpropyl modified poly(ethyleneimine)) surface dishes in comparison with uncoated titanium surface controls (Ti-Ref). The cell culture samples were processed in quatruplicate. In the 24 hour control group one array was suspended becauese of quality issues.