Salerno et al Progenesis exported protein list.xlsx

To assess the hierarchical importance of multipotent differentiation compared with trophic/immunoregulatory functions of MSCs, we have undertaken a detailed analysis of how these properties change with in vitro ageing of the cells. Using in vitro differentiation and repair models and integrating transcriptomics and proteomics data, we show that the multipotent differentiation capacity of MSCs falls with increasing passage, whereas trophic repair and immunoregulatory potency are maintained. In total four cell lines were profiled using LC-MSon a QExactive HF quadrupole-Orbitrap mass spectrometer coupled to a Dionex Ultimate 3000 RSLC nano-liquid chromatograph. Cell lines were isolated from four individuals. Secretome was collected from each cell line at passage 1, 5, 10 and 15 for a total of 16 samples.