Aberrant expression of interleukin-23-regulated miRNAs in T cells from patients with ankylosing spondylitis

Among the miRNAs regulated by IL-23, expression levels of 5 miRNAs were increased in T cells from patients with AS. The transfection of miR-29b-1-5p mimic could inhibit the IL-23-mediated STAT3 phosphorylation and might play a negative feedback control in the immunopathogenesis of AS. K562 cells, a human erythroleukemia line, purchased from the American Type Culture Collection (Manassas, USA) were cultured in medium with or without the presence of IL-23 (20 ng/mL) for 3 days. These cells were used for subsequent analysis.