Metagenome-assembled genomes (MAGs)

The MetaWRAP pipeline was employed for genome assembly and binning. Specifically, MEGAHIT was used to assemble sequences and generate contigs. Prodigal was used to predict open reading frames (ORFs) in each contig. MetaBAT2 and MaxBin2 were used to perform genome binning of assembled contigs, and the assembled bins were subsequently verified with the Bin_refinement module. The consolidated bins were improved by the Reassemble_bins module to generate final metagenome-assembled genomes (MAGs).

本研究采用MetaWRAP分析流程进行基因组组装与分箱分析。具体而言，首先使用MEGAHIT对测序序列进行组装并生成重叠群（contig）；随后利用Prodigal预测每个重叠群中的开放阅读框（open reading frames, ORFs）。采用MetaBAT2与MaxBin2对组装得到的重叠群执行基因组分箱操作，并通过Bin_refinement模块对所得分箱结果进行验证。最后通过Reassemble_bins模块对合并后的分箱结果进行优化，以生成最终的宏基因组组装基因组（metagenome-assembled genomes, MAGs）。