Comparative transcriptomic analysis ofcirculatingand muscle resident fibroadipogenic progenitors in middle cerebral artery occlusion (MCAO) mice.

Patients surviving stroke usually experience rapid muscle wasting and an increased risk of physical disability. Although multifactorial interactions including malnutrition, disuse, systemic catabolic imbalance and neurohormonal dysregulation are considered to contribute to the progression of stroke-related sarcopenia, the underlying mechanisms of this brain-muscle crosstalk remain elusive. Muscle-resident fibro/adipogenic progenitors (FAPs) are indispensable for maintaining muscle homeostasis and function as initial sensors of external perturbations. In the present study, we illustrate that FAPs rapidly respond to the overactive sympathetic nervous system (SNS), and egress from the muscle niche into circulation during the acute phase of stroke. In our study, we performed comparative transcriptomic analysis of circulating fibroadipogenic progenitors and muscle resident fibroadipogenic progenitors. Overall design: RNA-Seq was performed from RNA samples extracted from fibroadipogenic progenitors obtained from peripheral blood and muscle of MCAO mice.