Expression data of progesterone regulated miRNAs in U251 cells, derived from human glioblastoma

Glioblastoma multiforme (GBM), a grade IV astrocytoma, is the most common and aggressive brain tumor in adults, characterized by being highly infiltrative, angiogenic, and resistant to chemotherapy and radiotherapy. In previous works, has been determined that progesterone P4 increases proliferation, migration and invasion of cells derived from human GBMs through the interaction with its intracellular receptor (PR). In breast cancer, there exist evidence that P4 regulates the expression of miRNAs with tumor suppressor or oncogenic action, via the classical PR. The signature of miRNAs affected by P4 treatment in cells derived from human GBMs has not been determined. Therefore, we studied the effect of P4 on miRNAs expression pattern in U251 cells derived from a human GBM. Four cell cultures were performed for the total RNA extraction: U251 cell treated with P4, RU486, P4+ RU486 and vehicle (cyclodextrin) for 6 h. The hybridization on Affymetrix microarrays was carried out in triplicate for each treatment.