Notes: The strains (5-mm mycelial blocks) were grown on CM medium for 8 days, and the diameter of colonies was then measured (growth) or conidia were collected and counted (conidiation). 40-µl (1×105
Strains of Bacillus anthracis with knockout of specific genes or regions were constructed to analyze the contribution of those genes or regions to specific phenotype. Deletion was confirmed by sequenc
