Auxenochlorella pyrenoidosa genome annotation and protein sequences

A. pyrenoidosa RLXCh3 is a novel germplasm isolated from soil, with 63.65% protein content and 82 g/L of dry weight biomass. To achieve biofortification based on RLXCh3, PacBio II/IIe sequencing, alongside Hi-C sequencing and full-length transcriptome analysis, were performed to construct an improved chromosome-level haplotypic genome assembly consisting of 12 chromosomes and a genome size of 53.07 Mbp. Scaffold N50 (4.94 Mbp) and contig N50 (1.74 Mbp) were obtained, with a total of 12,091 protein-coding genes predicted. Additionally, genome-wide localizations of key genes on chromosomes involved in seven metabolic and regulatory pathways, such as TOR signaling pathway, fatty acid metabolism, selenocompound metabolism, were characterized. In summary, we acquired excellent A. pyrenoidosa isolates and reported the first chromosome-scale map of A. pyrenoidosa, providing a valuable resource for in-depth commercial protein bioproduction and nutrient biofortification.