Additional file 1 of Low-dose X-ray enhanced tumor accumulation of theranostic nanoparticles for high-performance bimodal imaging-guided photothermal therapy

Additional file 1: Fig. S1. (a) Hydrodynamic size changes of HAuNP@DTTC nanoparticles in different physiological solutions (H2O, PBS, FBS, DMEM + 10 % FBS) for 7 days; (b) Corresponding photographs of the first and seventh day; (c) Zeta potential of hollow Au, hollow Au with DTTC complex (Hollow Au/DTTC), and HAuNP@DTTC nanoparticles in DI water. Fig. S2. In vitro photoacoustic imaging of HAuNP@DTTC as PA contrast agents. (a) Combined ultrasound and photoacoustic images of PA phantom images under 808 nm excited laser for HAuNP@DTTC diepersion with different Au concentrations (from 0 to 20 OD). (b) Average PA signal as a function of wavelength and nanoplatforms concentration. Fig. S3. SERS spectra of Hollow Au-coupled DTTC (HAuNP@DTTC) nanoplatform in PBS. Fig. S4. Cell viabilities of 4T1 tumor cells incubated with HAuNP@DTTC with different concentrations for 24 h. Fig. S5. TEM micrographs of normal tissue (left) and tumor tissue (right). The red arrows denoted the HAuNP@DTTC. Fig. S6. Anti-tumor effect in vivo. Photographs of the 4T1-bearing mice at different time points after the various treatment. Fig. S7. Photographs of mice blood incubated with HAuNP@DTTC (1.6-200 μg mL -1) and corresponding percent hemolysis; water and PBS as the positive and negative controls, respectively. Table S1. The tumor growth inhibition (TGI) values of the 4T1-bearing mice of different groups.