Immunoenhancing effects of Montanide adjuvant on recombinant coccidia antigen vaccination against Eimeria spp. and comparative microarray analysis of intestinal lymphocytes

This study utilized comparative global gene expression microarray analysis to evaluate the effects of two different Montanide adjuvants, ISA70 and ISA71, in promoting chicken protective immunity following vaccination with profilin, an actin-regulatory protein that is expressed by multiple Eimeria species. Overall design: Three-condition experiment with reference hybridization design, 1) ISA70 plus profilin immunized chickens vs. profilin alone and 2) ISA71 plus profilin vs. profilin alone, 2 biological replicates with dye-switching, total 4 replicates for each adjuvants

本研究采用比较全球基因表达微阵列分析，以评估两种不同的蒙坦尼佐剂（ISA70和ISA71）在鸡接种疫苗后促进鸡的保护性免疫的作用，该疫苗为角蛋白（profilin），一种由多种艾美球虫种系表达的肌动蛋白调节蛋白。总体设计：包含参考杂交设计的三个条件实验，1）ISA70与角蛋白免疫接种的鸡与单独接种疫苗的角蛋白鸡进行比较，2）ISA71与角蛋白免疫接种的鸡与单独接种疫苗的角蛋白鸡进行比较，每个佐剂条件进行两次生物学重复，共计4个重复。