Contaminated sediment in the Detroit River provokes acclimated responses in wild brown bullhead (Ameiurus nebulosus) populations

In a previous study, adaptive responses to a single polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), were identified in brown bullhead (Ameiurus nebulosus) captured from contaminated sites across the Great Lakes. The tumor suppressor p53 and phase I toxin metabolizing CYP1A genes showed a protective and refractory response, respectively, up to the F1 generation (Williams and Hubberstey, 2014). As an extension to the first study, bullhead were exposed to sediment collected from sites along the Detroit River to see if these adaptive responses are attainable when fish from a contaminated site are exposed to a mixture of contaminants, instead of a single compound. p53 and CYP1A proteins were measured in both studies with the addition of phase II glutathione-s-transferase (GST) activity in the second. Three treatment groups were measured: acute (treated immediately), cleared (depurated for three months and subsequent treatment), and farm raised F1 offspring. All three treatment..., Brown bullhead (Ameiurus nebulosus) were collected from two reference 'clean' (Peche Island, Belle River) and contaminated sites (Trenton Channel, Belle Isle) in the Great Lakes and exposed to clean (Peche Island) and contaminated (Trenton Channel) sediment for 24 or 96 hours to confirm previously identified adaptive properties between different bullhead  populations in the Detroit River. Instead of exposing fish to a single polycyclic aromatic hydrocarbon (Benzo[a]pyrene) as in the previous study, we exposed fish to either clean or contaminated sediment from sites where fish were collected using a petite ponar.
Liver tissue samples were preserved in RNA later or flash frozen in liquid nitrogen at time of collection. 
Differences in CYP1A and p53 protein expression (phase I metabolism) and glutathion-s-transferase (phase II) enzyme activity was measured and quantified in F1 farm-raised, Acute and Cleared (for 3 months) fish. Protein expression in liver tissue was measured using western ..., , **Contaminated Sediment in the Detroit River selects for evolved CYP1A and p53 responses in wild brown bullhead (Ameiurus nebulosus) populations**
Access this dataset on Dryad: doi:10.5061/dryad.9p8cz8wn9
The dataset contains three workbooks:

1\. Western blot densitometry values for p53\\, CYP1A and loading control actin protein expression in brown bullhead (*Ameiurus nebulous*) fish retrieved from four sites in the Great Lakes (Pêche Isle (PI; 42o20’38”N, 82o55’43”W), Belle River (BR; 42o17’39”N, 82o42’43”W), Trenton Channel (TC; 42o10’33”N, 83o9’16”W), Belle Isle (BI; 42o20’57”N, 82o58’31”W). Fish were exposed to two classes of sediment: contaminated sediment and clean sediment. Contaminated sediment was collected from Trenton Channel (Detroit River, USA) and clean sediment was collected from Pêche Island (Detroit River, USA). Three categories of fish were exposed to both classes of sediment for two time points before they were sacrificed: 24 and 96 hours. Acute fish were taken direc...