Salivary apyrase in two P. orientalis colonies originated from Melka Werer (MW) and Addis Zemen (AZ), Ethiopia.

Phlebotomus papatasi was used as the control.*One unit of enzyme activity is defined as the amount of enzyme that releases one micromole of orthophosphate per minute from the nucleotide substrate at specified assay conditions.**Individual specific activity was calculated per gland pair as P. papatasi is characterized by dissimilar size of salivary glands [45].