One, two or three? Integrative species delimitation of short-range endemic Hemicycla species (Gastropoda: Helicidae) from the Canary Islands based on morphology, barcoding, AFLP and ddRADseq data

Hemicycla mascaensis and H. diegoi are short-range endemics that occur allopatrically in small areas in the Teno Mountains in the western part of Tenerife (Canary Islands). Both taxa have been recognised as distinct species based on differences in shell morphology and genital anatomy. Preliminary molecular analyses using mitochondrial markers suggested a potential paraphyly of H. diegoi with regard to H. mascaensis. We here use multilocus AFLP data and ddRADseq data as well as distribution data, data on shell morphology and genital anatomy to assess the status of these taxa using phylogenetic analyses, species tree reconstruction, and molecular species delimitation based on the multispecies coalescent as implemented in BFD* and BPP in an integrative approach. Our analyses show that, based on the analysis of multilocus data, the two taxa are reciprocally monophyletic. Species delimitation methods, however, tend to recognise all investigated populations as distinct species, albeit neither..., , , # Data from: One, two or three? Integrative species delimitation of short-range endemic Hemicycla species (Gastropoda: Helicidae) from the Canary Islands based on morphology, barcoding, AFLP and ddRADseq data

[https://doi.org/10.5061/dryad.rr4xgxd7k](https://doi.org/10.5061/dryad.rr4xgxd7k)

## Description of the data and file structure

### Files and variables

#### File: Data\_set\_01\_Hemicycla\_COI1\_16S.nexus

**Description:** Aligned partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (*cox1*) (alignment positions 1-655) and 16S rRNA (alignment positions 656-1,488) genes in nexus format.

#### File: Data\_set\_02\_AFLP.nex

**Description:** AFLP loci obtained from 15 specimens belonging to the genus *Hemicycla* in nexus format.

#### File: Data\_set\_03\_AFLPred.nex

**Description:** AFLP loci obtained from 9 specimens belonging to the *Hemicycla* *mascaensis* complex in nexus format.

#### File: Data\_set\_4\_RADseq\_SNP\_all\_polymorphic\_positions.nex

**Desc...