Non-cell autonomous control of liver autophagy via nutrient sensing AgRP neurons

Here, we revealed that optogenetic activation of AgRP neurons alters liver transcriptome associated with autophagy, glucose metabolism, lipid metabolism and ß-oxidation. Overall design: 6mm Optic fibers were placed above the Arcuate Nucleus of adult AgRP-IRES-Cre wt/wt ROSA26 ChR2 fl/wt and AgRP-IRES-Cre tg/wt ROSA26 ChR2 fl/wt mice. Further, mice received either 2 hours or 4 hours of photosimulation in the light cycle in the absence of food.