Effect on low dose stress on the miRNA levels in Drosophila melanogaster.

The young adult males from D. melanogaster subjected to mild perturbation in environmental conditions. The adult flies were subjected to a temporary heat stress (HS), where the test subjects were moved to an incubator set at 32 0C for 2 hours. The HS flies were placed at 22 0C under standard conditions for 1 hour. This is the heat primed condition. The heat primed were then transferred to vials with thin strips of Whatman paper soaked with 5% hydrogen peroxide in 30 % sucrose solution at 22 0C under standard conditions for 18 hours. This is the combined stress condition. The miRNA expression was studied after the primed, combined stress and age-matched no stress control conditions. The mRNA expression was also studied for primed and combined stress conditions. The sequencing was performed on a Illumina Nextseq500.