RNA-seq of in vivo B7H3_CAR_T cells loaded with oncolytic virus VSVΔ51

To investigate the in vivo functions of CR2/3-CAR-T loaded with VSVΔ51 after infiltrating tumor microenvironment, we analyzed the transcriptomic signatures at early stage after CAR-T infiltrating into tumor tissue. We isolated the CAR-T cells from the mouse tumor and used RNA sequence to study the global gene expression. We identified differentially expressed genes in B7H3-CAR-T cells and CR2/3-B7H3-CAR-T cells loaded with loading VSVΔ51, aiming to identify the changed pathways that are influenced by the cross-connection between VSV-G protein and CR2/3-CAR moiety. For CAR-T cell construction, the activated CD3 positive human T lymphocytes were transduced with lentivirus carrying the B7H3-CAR and CR2/3-B7H3-CAR. To preload VSVΔ51, B7H3-CAR-T or CR2/3-B7H3-CAR-T cells were co-incubated with VSVΔ51 (MOI=10) on ice for 1 hour. The CAR-T cells were injected into the NCG mice bearing U87 tumor, then CAR-T cells were isoalted from the tumor tissue after 16 house post injection. The samples used in the RNA sequence analysis were in triplicates.