RNA-seq profiling of C/EBPß-overexpressing myoblasts [proliferating myoblasts]

We previously identified C/EBPß as an inhibitor of myogenic differentiation and a regulator of muscle satellite cell self-renewal. We found that C/EBPß is regulated during the transition from proliferation to growth arrest in myoblasts and overexpression of C/EBPß in myoblasts promotes cell growth arrest in vitro and improves engraftment of satellite cells into the stem cell niche in vivo. To identify the molecular mechanism by which C/EBPß regulates myoblast proliferation and growth arrest, we performed RNA-seq on C/EBPß-overexpressing myoblasts. Overall design: mRNA profiles of C/EBPß-overexpressing C2C12 cells and empty vector controls in growth media.