10X multiome RNA+ATAC sequencing of pooled samples from 5 locations of the adult lung

To capture the transcriptional diversity and epigenetic regulation of single cells across different parts of the lung, we profiled adult lung samples from 5 human donors and 5 locations each (trachea, bronchi, parenchyma lower left lobe, parenchyma upper left lobe, parenchyma and small airways from lower left lobe). The 25 samples have been pooled into 5 pools with distinct donor and location combinations. Three libraries from each of the 5 pools have been sequenced, resulting in 15 samples in total.