Histone H3.3K27M mutation activates multiple cancer/testis antigens

Lysine27Methionine mutations (K27M) in the histone H3 (H3.3 and H3.1) are highly prevalent in pediatric high-grade gliomas (HGG). This study found H3.3K27M caused the upregulation of multiple cancer/testis (CT) antigens, include IL13RA2 and VCX family proteins. Overexpression of VCX3A/B stimulates the expression of genes involved in immune response. Total RNA was extracted from Res259 cells stably expressing H3.3K27M, pCMV6-Entry (empty vector), different forms of GFP-VCX3A/B, or GFP (as control), and subjected to an Illumina HT12.2 Bead CHIP array analysis.