Additional file 2 of Conserved sequence motifs in human TMTC1, TMTC2, TMTC3, and TMTC4, new O-mannosyltransferases from the GT-C/PMT clan, are rationalized as ligand binding sites

Additional file 2. Positions of TM regions and the DUF1736 segment in TMTC sequences. The file AF2-2020-06-TMs-TMTCs.xlsx provides the sequence positions of 11 TM regions (maximum consensus region from 5 TM predictors DAS-tmfilter [43, 44], HMMTOP [45, 46], PHOBIUS [47, 48], TMHMM [49, 50] and TOPPRED2 [51, 52]) in various animal TMTC sequences (the same sequences used in the alignment of Fig. 1) as well as the sequence segments that correspond to the DUF1736 region. We also indicate the approximate core of the hydrophobic region in EL4/DUF1736 that gives rise to false-positive TM predictions but actually represents a helix in the ER lumen parallel to the ER membrane (column “helical hydrophobic region”) if the TM predictors detect any. To emphasize, ‘exact boundaries’ of TM regions are difficult to derive with sequence-analytic methods; a flexibility of up to a few residues on either side should be considered as only the hydrophobic core of the TM is accurately predicted.